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Lab overview

Bioex is linked to the Graduate Program in Health Sciences (PPGCS) of the School of Medicine at the Pontifical Catholic University of Paraná (PUCPR) in Brazil. Our research aims to understand the biochemical and molecular mechanisms induced by physical exercise on the redox biology in diverse tissues and organs. We use advanced technologies and research techniques to explore and investigate the scientific hypotheses formulated in our Laboratory. Our research is supported by government funding and other foundations.


The Bioex team is composed of researchers, undergraduate and postgraduate students (master's and doctorate), post-doc and collaborators. Know more about the members of our 2022 team below.

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Head of Lab and main investigator

Ricardo A. Pinho, PhD

Dr. Pinho has an undergraduate and a Master's degree in Physical Education (UFSC, Brazil,1992/1999) and a Ph.D. in Biological Sciences - Biochemistry (UFRGS, Brazil, 2005).  Dr. Pinho is a professor of Graduate and undergraduate programs at the School of Medicine and Life Sciences at Pontificia Universidade Católica do Paraná (PUCPR), Brazil. In the Graduate Program in Health Sciences, Dr. Pinho is a professor and supervisor (MSc and Ph.D.), honorary professor at Ningbo University, China, and Head of the Biochemistry of Exercise Lab in Health. He is a member of the Society for Free Radical Research - Europe and a Member of the Advisory Board in the Area of Biological Sciences of the Araucária Foundation (Paraná, Brazil). Acts as a reviewer for several international journals and Associate Editor. Dr. Pinho has experience in the Biochemistry and physiology of exercise, mainly oxidative stress and pathophysiology of chronic degenerative diseases. (CNPQ scientist , index-h = 40).




Our projects aim to investigate the role of physical exercise and antioxidants on the redox response of degenerative diseases. Research in the  BIOEX involves a constant review of the scientific issues on exercise and health.The strength of our research lies in the breadth and depth of the experimental approaches we use in understanding the molecular and biochemical mechanisms induced by physical exercise. Learn more about our current research projects below.

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The role of the Sodium-glucose cotransporter 2 (SGLT2) inhibitor and physical exercise in the regulation of the redox system and the inflammassoma complex in animals with obesity and experimental diabetic nephropathy

The results of our research are published in different international journals, which have been cited and recognized by other researchers. See some of our recent publications (2021/2022). You can find a complete list of our publications at Orcid:

Obesity-dependent molecular alterations in fatal COVID-19: A retrospective postmortem study of metabolomic profile of adipose tissue.

Pilger BI, Castro A, Vasconcellos FF, Moura KF, Signini ÉF, Marqueze LFB, Fiorenza-Neto EA, Rocha MT, Pedroso GS, Cavaglieri CR, Ferreira AG, Figueiredo C, Minuzzi LG, Gatti da Silva GH, Castro GS, Lira FS, Seelaender M, Pinho RA. J Cell Biochem. 2024 Apr 9. doi: 10.1002/jcb.30566.

Epigenetic and "redoxogenetic" adaptation to physical exercise. 

Radak Z, Pan L, Zhou L, Mozaffaritabar S, Gu Y, A Pinho R, Zheng X, Ba X, Boldogh I. Free Radic Biol Med. 2024 Jan;210:65-74. doi: 10.1016/j.freeradbiomed.2023.11.005.


The effects of long-term lactate and high-intensity interval training (HIIT) on brain neuroplasticity of aged mice. 

Lei Z, Mozaffaritabar S, Kawamura T, Koike A, Kolonics A, Kéringer J, Pinho RA, Sun J, Shangguan R, Radák Z. Heliyon. 2024 Jan 10;10(2):e24421. doi: 10.1016/j.heliyon.2024.e24421.


Bone-marrow mononuclear cells and acellular human amniotic membrane improve global cardiac function without inhibition of the NLRP3 Inflammasome in a rat model of heart failure.

Takejima AL, Machado-Júnior PAB, Blume GG, Simeoni RB, Francisco JC, Tonial MS, Marqueze LFB, Noronha L, Olandoski M, Abdelwahid E, Carvalho KAT, Pinho RA, Guarita-Souza LC. An Acad Bras Cienc. 2024 Mar 4;96(1):e20230053.

Carnosine supplementation and retinal oxidative parameters in a high-calorie diet rat model. 

de Almeida Torres RJ, Moreto F, Luchini A, de Almeida Torres RJ, Longo SP, Pinho RA, Nagashima S, de Noronha L, Ferron AJT, de Almeida Silva CCV, Correa CR, Aldini G, Ferreira ALA. BMC Ophthalmol. 2023 Dec 8;23(1):502. doi: 10.1186/s12886-023-03255-y.


Maternal exercise during pregnancy modulates genotoxicity caused by high fructose consumption in mice offspring.

Magenis ML, Damiani AP, de Oliveira Monteiro I, Dagostin LS, Dos Santos da Silva N, Scussel R, Nagashima S, Langie SAS, Pinho RA, de Andrade VM. Mutagenesis. 2023 Nov 29:gead035. doi: 10.1093/mutage/gead035.


Mild to moderate post-COVID-19 alters markers of lymphocyte activation, exhaustion, and immunometabolic responses that can be partially associated by physical activity level- an observational sub-analysis fit- COVID study. 

Silva BSA, Pereira T, Minuzzi LG, Padilha CS, Figueiredo C, Olean-Oliveira T, Dos Santos IVM, von Ah Morano AE, Marchioto Júnior O, Ribeiro JPJ, Dos Santos VR, Seelaender M, Teixeira AA, Dos Santos RVT, Lemos VA, Freire APCF, Dorneles GP, Marmett B, Olean-Oliveira A, Teixeira MFS, Seraphim PM, Caseiro A, Pinho RA, Islam H, Little JP, Krüger K, Rosa-Neto JC, Coelho-E-Silva MJ, Lira FS. Front Immunol. 2023 Sep 11;14:1212745. doi: 10.3389/fimmu.2023.1212745. eCollection 2023.


Voluntary exercise does not increase gastrointestinal motility but increases spatial memory, intestinal eNOS, Akt levels, and Bifidobacteria abundance in the microbiome.

Bakonyi P, Kolonics A, Aczel D, Zhou L, Mozaffaritabar S, Molnár K, László L, Kutasi B, Tanisawa K, Park J, Gu Y, Pinho RA, Radak Z. Front Physiol. 2023 Aug 16;14:1173636. doi: 10.3389/fphys.2023.1173636. eCollection 2023.


Regulation of Redox Profile and Genomic Instability by Physical Exercise Contributes to Neuroprotection in Mice with Experimental Glioblastoma. Marqueze LFB, Costa AK, Pedroso GS, Vasconcellos FF, Pilger BI, Kindermann S, Andrade VM, Alves ACB, Nery T, Silva AA Jr, Carvalhal SRS, Zazula MF, Naliwaiko K, Fernandes LC, Radak Z, Pinho RA. Antioxidants (Basel). 2023 Jun 26;12(7):1343. doi: 10.3390/antiox12071343.

A remote, fully oriented personalized program of physical exercise for women in follow-up after breast cancer treatment improves body composition and physical fitness. 

França-Lara ÉG, Weber SH, Pinho RA, Casali-da-Rocha JC, Elifio-Esposito S. Sports Med Health Sci. 2023 Mar 27;5(2):128-136. doi: 10.1016/j.smhs.2023.03.005.


Exercise friend or foe? for the management of oxidative stress in health and diseases 

Thirupathi A, Gu Y, Pinho RA. Front Physiol. 2023 May 25;14:1216566. doi: 10.3389/fphys.2023.1216566.


Acellular Biomaterials Associated with Autologous Bone Marrow-Derived Mononuclear Stem Cells Improve Wound Healing through Paracrine Effects. 

de Souza IC, Takejima AL, Simeoni RB, Gamba LK, Ribeiro VST, Foltz KM, de Noronha L, de Almeida MB, Neto JRF, de Carvalho KAT, da Silveira PCL, Pinho RA, Francisco JC, Guarita-Souza LC. Biomedicines. 2023 Mar 24;11(4):1003. doi: 10.3390/biomedicines11041003.


Autonomic Function Recovery and Physical Activity Levels in Post-COVID-19 Young Adults after Immunization: An Observational Follow-Up Case-Control Study. 

Freire APCF, Amin S, Lira FS, Morano AEVA, Pereira T, Coelho-E-Silva MJ, Caseiro A, Christofaro DGD, Dos Santos VR, Júnior OM, Pinho RA, Silva BSA. Int J Environ Res Public Health. 2023 Jan 27;20(3):2251. doi: 10.3390/ijerph20032251.


Lung Inflammasome Activation in SARS-CoV-2 Post-Mortem Biopsies.

Baena Carstens L, Campos D'amico R, Fernandes de Moura K, Morais de Castro E, Centenaro F, Silva Barbosa G, Vieira Cavalcante da Silva G, Brenny I, Honório D'Agostini JC, Hlatchuk EC, Pissette de Lima S, Camargo Martins AP, De Castro Deus M, Konzen Klein C, Kubaski Benevides AP, Nagashima S, Machado-Souza C, Pinho RA, Pellegrino Baena C, de Noronha L. Int J Mol Sci. 2022 Oct 27;23(21):13033. doi: 10.3390/ijms232113033.


Redox Status Is the Mainstay of SARS-CoV-2 and Host for Producing Therapeutic Opportunities. 

Thirupathi A, Gu Y, Radak Z, Pinho RA. Antioxidants (Basel). 2022 Oct 19;11(10):2061. doi: 10.3390/antiox11102061.


Physical Training Protects Against Brain Toxicity in Mice Exposed to an Experimental Model of Glioblastoma.

Costa AK, Marqueze LFB, Gattiboni BB, Pedroso GS, Vasconcellos FF, Cunha EBB, Justa HC, Baldissera AB, Nagashima S, de Noronha L, Radak Z, Fernandes LC, Pinho RA. Neurochem Res. 2022 Nov;47(11):3344-3354. doi: 10.1007/s11064-022-03685-y.


Resistance Exercise Training Improves Metabolic and Inflammatory Control in Adipose and Muscle Tissues in Mice Fed a High-Fat Diet. 

Effting PS, Thirupathi A, Müller AP, Pereira BC, Sepa-Kishi DM, Marqueze LFB, Vasconcellos FTF, Nesi RT, Pereira TCB, Kist LW, Bogo MR, Ceddia RB, Pinho RA. Nutrients. 2022 May 24;14(11):2179. doi: 10.3390/nu14112179.


Gold Nanoparticle-Based Therapy for Muscle Inflammation and Oxidative Stress. 

Pinho RA, Haupenthal DPS, Fauser PE, Thirupathi A, Silveira PCL. J Inflamm Res. 2022 May 31;15:3219-3234. doi: 10.2147/JIR.S327292. eCollection 2022.


Lung-Brain Crosstalk in Sepsis: Protective Effect of Prophylactic Physical Exercise Against Inflammation and Oxidative Stress in Rats. 

Giustina AD, Rodrigues JF, Bagio E, Bonfante S, Joaquim L, Zarbato G, Stork S, Machado RS, de Souza Goldim MP, Danielski LG, Mathias K, Dacoregio C, Cardoso T, Predroso GS, Venturini LM, Zaccaron RP, Silveira PCL, Pinho RA, Petronilho F. Mol Neurobiol. 2022 Jun;59(6):3860-3872. doi: 10.1007/s12035-022-02823-5.


Exercise Friend or Foe? For the Management of Oxidative Stress in Health and Diseases. 

Thirupathi A, Gu Y, Wiltshire HD, Pinho RA. Front Physiol. 2022 Mar 23;13:881197. doi: 10.3389/fphys.2022.881197.


Role of Body Mass and Physical Activity in Autonomic Function Modulation on Post-COVID-19 Condition: An Observational Subanalysis of Fit-COVID Study. 

Freire APCF, Lira FS, Morano AEVA, Pereira T, Coelho-E-Silva MJ, Caseiro A, Christofaro DGD, Marchioto Júnior O, Dorneles GP, Minuzzi LG, Pinho RA, Silva BSA. Int J Environ Res Public Health. 2022 Feb 21;19(4):2457. doi: 10.3390/ijerph19042457.

Collagen matrices are preserved following decellularization of a bovine bone scaffold. 

Dantas LR, Ribeiro VST, Kraft L, Pinho RA, Suss PH, Vasconcellos FTF, de Noronha L, Tuon FF.Cell Tissue Bank. 2022 Sep;23(3):531-540. doi: 10.1007/s10561-021-09987-9.


Effects of the intranasal application of gold nanoparticles on the pulmonary tissue after acute exposure to industrial cigarette smoke. 

Porto GD, Haupenthal DPDS, Souza PS, Silveira GB, Nesi RT, Feuser PE, Possato JC, de Andrade VM, Pinho RA, Silveira PCL. J Biomed Mater Res B Appl Biomater. 2022 Jun;110(6):1234-1244. doi: 10.1002/jbm.b.34994.


Role of mononuclear stem cells and decellularized amniotic membrane in the treatment of skin wounds in rats.

Takejima AL, Francisco JC, Simeoni RB, de Noronha L, Garbers LAFM, Foltz KM, Junior PABM, Souza IC, Pinho RA, Carvalho KAT, Guarita-Souza LC. Tissue Barriers. 2022 Apr 3;10(2):1982364. doi: 10.1080/21688370.2021.


Older Adults with Pre-sarcopenia/Sarcopenia have Increased Gastrocnemius Muscle Activity to Maintain Upright Postural Control at Stance Perturbation. 

Vendramini, I.C., Papcke, C., da Veiga Neto, P.S. et al. J. Med. Biol. Eng. 43, 437–445 (2023).

The Role of SIRT3 in Exercise and Aging. Zhou L, Pinho R, Gu Y, Radak Z. Cells. 2022 Aug 20;11(16):2596. doi: 10.3390/cells11162596.


Lab Symposium

Symposium of Exercise Biochemistry in Health


The Symposium of Exercise Biochemistry in Health is an event promoted by Bioex to disseminate scientific advances related to physical exercise's biochemical and molecular effects on human health. The event is held annually at PUCPR and has the participation of important researchers. The next event will be 7 to 9 November 2024 

A little of our scientific and social routine

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Contact us

Thank you for your interest in our research. Please contact us if you have any questions or comments about our work and publications, or, if you wish to try out for a master´s degree, doctorate or a post-doc position. We would love to hear from you.

Adress: R. Imac. Conceição, 1155 - Prado Velho, Curitiba - PR, 80215-901, Brazil

Phone: (+55 48) 32716333

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